Nitric Oxide Synthase (Part II)



Nitric Oxide Synthase (NOS) is an enzyme which catalyzes the production of the signaling molecule NO from arginine. In mammals, NOS appears as 3 isozymes: neuronal NOS (nNOS), cytokine-inducible NOS (iNOS) and endothelial NOS (eNOS). The N-terminal domain of NOS is an oxygenase domain (OD). NOS cofactors are: NADPH, FAD, FMN, heme and O2. The images at the left and at the right correspond to one representative NOS, i.e. the crystal structure of Nitric Oxide Synthase from Bacillus subtilis (2amo).

Endothelial NOS
3nld, 3nle, 3nlf, 3nlg, 3jww, 3jwx, 3jwy, 3jwz, 3dqs, 3dqt – b-eNOS OD+pyrrolidinomethyl aminopyridine derivative – bovine

1dm6 – b-eNOS OD+guanidine derivative

3nlh – b-eNOS OD (mutant)+pyrrolidinomethyl aminopyridine derivative

3e7s – b-eNOS OD+inhibitor

1rs8, 1rs9 – b-eNOS OD +dipeptide inhibitor

1nse – b-eNOS OD

1p6n – b-eNOS OD +dipeptide inhibitor

5nse, 1fol, 1foo, 1fop, 1dm7, 2nse, 3nse, 4nse – b-eNOS OD+arginine derivative

6nse – b-eNOS OD+canavanine

7nse – b-eNOS OD+ADMA

1i83, 1d1v, 1d1x, 1d1y, 1ed4, 9nse – b-eNOS OD+urea derivative

1dmj, 1dmk – b-eNOS OD+pterin derivative

1foi, 1dmi – b-eNOS OD+inhibitor

1d0c, 8nse, 1foj, 1ed5 – b-eNOS OD+indazole derivative

1dm8 – b-eNOS OD+triazole derivative

1ed6 – b-eNOS OD+NO

1q2o – b-eNOS OD (mutant)+dipeptide inhibitor

1p6l, 1p6m – r-eNOS OD +dipeptide inhibitor

3eah – h-eNOS OD - human

3nos – h-eNOS OD+arginine

1m9j – h-eNOS OD+chlorzoxazone

1m9k, 1m9r – h-eNOS OD+indazole derivative

Neuronal NOS
2hx2 – b-nNOS OD+nitroguanidine derivative

3nli, 3nlj, 3nlk, 3nln, 3nlo, 3nlp, 3nlq, 3nlr, 3jws, 3jwt, 3jwu, 3jwv, 3jx0, 3jx1, 3jx2, 3jx3, 3jx4, 3jx5, 3jx6, 3dqr – r-nNOS OD (mutant)+pyrrolidinomethyl aminopyridine derivative – rat

3nlm, 3nny, 3nnz, 3n2r, 3b3o, 3b3p, 3b3m, 3b3n – r-nNOS OD+pyrrolidinomethyl aminopyridine derivative

2hx3 – r-nNOS OD+nitroguanidine derivative

2hx4, 1zvl – r-nNOS OD+arginine derivative

1om4, 1k2r, 1k2s, 1lzx – r-nNOS OD+arginine derivative

1k2t, 1k2u – r-nNOS OD+thiourea derivative

1om5 – r-nNOS OD+indazole derivative

1lzz, 1m00 – r-nNOS OD+guanidine derivative

3fc5, 1zvi – r-nNOS OD

1qw6 – r-nNOS OD+arginine derivative

1qwc – r-nNOS OD+inhibitor

3jt3, 3jt4, 3jt5, 3jt6, 3jt7, 3jt8, 3jt9, 3jta – r-nNOS OD+ornithine derivative

3hsn – r-nNOS OD+NHA+CO

3hso, 3hsp - r-nNOS OD+NHA+NO

2g6h, 2g6i, 2g6j – r-nNOS OD+pterin derivative

2g6k, 2g6l – r-nNOS OD+pterin derivative+NO

2g6m, 2g6n, 2g6o – r-nNOS OD+pterin derivative+CO

1zzq, 1zzr, 1zzs, 1zzt, 1zzu – r-nNOS OD (mutant)+dipeptide inhibitor

1rs6, 1rs7, 1p6h, 1p6i, 1p6j – r-nNOS OD +dipeptide inhibitor

1mmv, 1mmw – r-nNOS OD + inhibitor

1p6k – r-nNOS OD (mutant)+dipeptide inhibitor

1vag – m-nNOS OD+inhibitor - mouse

Inducible NOS
3nqs, 3e65 – m-iNOS OD + pterin derivative

1jwj, 1jwk – m-iNOS OD (mutant)+ pterin derivative

2bhj – m-iNOS OD+coumarin

3eai, 3ebd, 3ebf, 1r35, 2nod – m-iNOS OD+pterin derivative

3nod – m-iNOS OD+product analog

1noc – m-iNOS OD+chloramphenicol acetyltransferase

3e67 – m-iNOS OD+4-MAP

3e68, 3e6l, 3e6n, 3e6o, 3e6t, 3e7i, 3e7m, 3e7t, 1vaf, 1qw5, 1dd7 – m-iNOS OD+inhibitor

2orq, 2orr, 2ors, 2ort, 2oro, 2orp, 1nos – m-iNOS OD+imidazole derivative

3dwj – m-iNOS OD (mutant)

1qw4, 1dwv, 1dww, 1dwx, 1nod – m-iNOS OD+arginine derivative

2nos – m-iNOS OD+guanidine derivative

1n2n – m-iNOS OD+CN

1m9t – m-iNOS OD+indazole derivative

1m8d – m-iNOS OD+chlorzoxazone

1m8e, 1m8h, 1m8i, 1m9m, 1m9q – m-iNOS OD+ indazole derivative

3hr4 – h-iNOS residues 503-715+calmodulin – human

3e7g – h-iNOS residues 82-505+inhibitor

3ej8 – h-iNOS OD+imidazole

4nos – h-iNOS OD+inhibitor

4nsi – h-iNOS OD+arginine

2nsi – h-iNOS OD+thiourea derivative

Prokaryote NOS
2amo – BsNOS – Bacillus subtilis

2an0, 2an2 – BsNOS (mutant)

2fbz, 2fc2, 2fc1 – BsNOS+NO

1m7v, 1m7z – BsNOS+THF+arginine

2flq – NOS+arginine – Geobacillus stearothermophilus